(1160-A) Gravity Assisted Processing of Whole Blood for Amphetamine Analysis in a Point of Care Limited Access Facility

Abstract: Liquid Chromatography tandem Mass Spectrometry has garnered attention in recent times due to its versatile range of applications. However, limitations still exist when it comes to effective sample preparation methods. Current methods often have limitations due to limited poor analyte recovery, especially when dealing with a complicated matrix like whole blood. Microfluidic techniques can be exploited to address this issue, and can introduce simplification of the workflow when it comes to LC-MS/MS based translational research especially in areas of limited resources. Herein, we have designed and developed a low cost microfluidic chip that utilizes the principles of capillary flow for a faster sample preparation of whole blood for the detection of Amphetamine, a drug of abuse, without needing elaborate technologies for immediate analyte extraction. Even though the analyte will be eventually quantified using an in-house developed LC-MS/MS assay, the device allows for enhanced flexibility and simplicity by providing a straightforward but valuable technique to collect and prepare samples that can later be directed to other areas where quantification is more feasible if need be.