(1029-B) Characterization of membrane protein using mass spectrometry-based technologies — affinity selection-mass spectrometry (AS-MS)
Monday, February 5, 2024
2:00 PM – 3:00 PM EST
Location: Exhibit Halls AB
Abstract: Affinity Selection-Mass Spectrometry (AS-MS) is an emerging technology in drug discovery. In practice, its primary focus is on discovering binders for target molecules. As reported publicly, AS-MS is employed for hit finding in high-throughput screening campaigns for various target molecules, ranging from soluble proteins to challenging targets. Recently, it has been applied for binder exploration of functionally challenging target molecules, such as membrane proteins, RNA, and proteins of interest involved in targeted protein degradation. Even membrane proteins can be utilized in AS-MS technology, along with soluble target molecules. However, in many cases, proper sample preparation is necessary for membrane proteins, which may involve solubilization with mild detergents to maintain their proper folding. In this poster presentation, we demonstrate that AS-MS, using membrane fractions or microsome as protein material, is useful not only for evaluating binding affinity but also for profiling the inhibition mode of membrane proteins and structure determination of membrane protein complex with inhibitor.