(1279-D) Enhancing Pathogen Surveillance with Automated NGS Library Preparation

Abstract: With the rapid growth of Next Generation Sequencing (NGS), having a robust automation-friendly library preparation solution is a minimum requirement for the handling of precious samples across many applications. Automating library preparation offers benefits in throughput and consistency. However, the transition from manual workflows to automation can often be daunting and time-consuming, with concerns around the time needed to develop automated protocols and dead volume requirements. This poster describes TGen North’s process of transitioning to an automated workflow using SPT Labtech’s firefly® along with the Watchmaker DNA Library Prep Kit with Fragmentation.

The HIVE Laboratory at TGen North plays a pivotal role in Arizona's pathogen surveillance initiatives, significantly aiding public health efforts and supporting underserved communities. By employing whole genome sequencing, the lab adeptly tracks emerging outbreaks, fostering well-informed public health responses. The demand for high-throughput sample processing and precise pathogen surveillance raised challenges in their manual NGS library preparation, with risks of human error and variability among lab technicians.

To address these challenges, TGen North considered automated liquid handling systems from various providers. They selected firefly for its versatility and efficient use of laboratory space, combining functionalities for 96/384-well pipetting, non-contact dispensing, shaking, and incubating in a compact benchtop design. The choice was further influenced by the system’s intuitive and user-friendly software.

The team rigorously evaluated seven DNA library preparation kits, ultimately opting for the Watchmaker DNA Library Prep Kit with Fragmentation. This kit stood out for its superior conversion rates and a streamlined automation-friendly workflow, compatible with PCR-free options.

The adoption of automated library preparation has significantly enhanced TGen North's capabilities. The new system offers greater flexibility, minimizes reagent wastage, and enables more effective handling of valuable samples. Consequently, this advancement has bolstered TGen North’s capacity for timely pathogen surveillance, while also freeing resources for more complex laboratory tasks.