(1187-D) Automation of Illumina DNA PCR-Free Prep, Tagmentation Library Construction Kit with Plate-based Unique Dual Indices on the Biomek NGeniuS Next Generation Library Prep System

Abstract: The Biomek NGeniuS Next Generation Library Prep System from Beckman Coulter Life Sciences is a reliable, easy-to-use, purpose-built liquid handler for NGS library preparation ideal for laboratories requiring more flexibility and less hands-on time. The user-friendly software requires no sophisticated programming skills, making it easy to set up and run. The instrument’s hardware features include an on-deck thermal cycler, gripper, temperature-controlled reagent storage, and high-density tip storage, enabling more walkaway time. An extensive and growing library of complimentary demonstrated NGS sample prep protocols allow the user to select from a variety of applications for next generation sequencing workflows. One such workflow is the Illumina DNA PCR-Free Prep, Tagmentation Library Construction kit. It provides researchers with the ability to create sequence ready libraries without the use of Polymerase Chain Reaction (PCR), which can introduce bias by swamping out tumor-normal variants and can result in uneven coverage during Whole Genome Sequencing (WGS).
In this poster, we show the utility of using the Biomek NGeniuS Next Generation Library Prep System to perform the Illumina DNA PCR-Free Prep, Tagmentation library construction process, which saves researchers valuable hands-on time and offers a highly streamlined setup process for users who are new to liquid handling automation.