(1207-D) Accelerating Drug Discovery at Totus Medicines: DNA-Encoded Library Screening with firefly®

Abstract: DNA-encoded libraries (DELs) have emerged as a transformative technology within drug discovery. The exploration of large libraries of small molecule ligands as a collective rather than an individual has springboarded DELs to the forefront of high throughput screening. The consistent and stringent nature of DEL screening lends itself easily for translation from manual processing to automation, providing significant advantages including reproducibility, workflow optimizations, and scalability. Furthermore, the use of automated instrumentation supplies the engineering tools required for rapid, simultaneous screening of billions of compounds against multiple, diverse biological targets.  



Building upon traditional DEL methods, Totus Medicines has developed a platform capable of identifying covalent small molecule ligands via high-throughput cell-based DEL screening. This poster outlines how Totus Medicines is streamlining hit identification, optimization, and technology development, to accelerate the discovery of novel therapeutic candidates using SPT Labtech’s firefly automated liquid handling platform. We’ll discuss metrics they’ve collected over 1 year of screening using their proprietary covalent DELs to run over 2000 unique selections against more than 100 oncology targets.