Phenotypic screening is experiencing a Renaissance in the pharmaceutical industry, based on its successful track record in delivering first-in-class medicines stemming from novel biology. Although phenotypic screening may appear at first sight to be similar to target-based screening, there are fundamental differences between these two approaches. This course will cover a range of relevant topics with a goal of providing practical information to help prosecute phenotypic projects more effectively from assay design all the way to clinical trials.