CytoTronics has developed a state-of-the-art semiconductor 96 microplate platform designed for high-resolution, real-time, multi-parametric electrical “imaging” of cells. Each well in the microplate boasts an array of >100,000 electrochemical electrodes spaced at 12.5 µm pitch, enabling single-cell resolutions. The platform uniquely offers three live-cell modalities –impedance, electrochemical and electrophysiological, all utilizing the same electrode array. The measurements are label-free, non-invasive, and non-destructive, facilitating continuous cell monitoring through growth, pharmacological modulation, and cell death over days or weeks. Combining the modalities into a single microplate generates high-dimensional, live cell datasets, capturing >20 functional and morphological parameters, including tissue barrier, cell-surface attachment, cell flatness, and motility through unique field-based impedance measurements. Electrochemical measurements offer insight into redox states and cell metabolism, while electrophysiological techniques enable functional assessments of electrogenic cells like cardiomyocytes and neurons. All modalities harness the 12.5 µm spatial resolution and allow measurements at intervals ranging from minutes to hours, creating electrical “heat-map” images and time-lapsed videos revealing the end-to-end journey of the experiment. Further insights are derived from spatial image and time-domain analyses. Manipulation capabilities complement the measurements including electrochemical permeabilization, cell patterning, and electrical stimulation for exciting cardiac cells or neurons. These extensive capabilities are integrated into a standard-sized microplate, serving as a self-contained measurement device, capable of measuring over 30 types of cells, ranging from epithelial and mesenchymal cancer cells to suspension cell lines, primary rodent cells, and human induced pluripotent stem cells (iPSCs). During experiments, each semiconductor microplate seamlessly interfaces with readers, with slots for 1 to 8 plates at a time – with the potential to scale and integrate with high throughput screening automation. An upcoming release of a 384-well format, compatible with the same readers, will further expand high-throughput capabilities.