Abstract: Robotics were introduced into pharmaceutical R&D laboratories in the 1980’s. A broader utilization of the technology occurred across labs in the 1990’s primarily due to the adoption of a standard for the microwell plate by the Society of Biological Screening (progenitor organization of the Society of Laboratory Automation and Screening). The 1st generation of laboratory robots are either standalone systems or a collection of 2 or more systems/devices that are permanently physically integrated into a single robotic workflow. Usually, 1st generation integrated systems have a master system that controls all the other systems. Significant development of 1st generation laboratory robots was driven by the need of pharmaceutical companies to automate screening of small molecule libraries to identify potential small molecule drug candidates. In the 2010’s, a 2nd generation of laboratory robotics were introduced that increased the number of options for screening purposes by enabling modular physical integrations of multiple systems. The 2nd generation robot was made possible by the development of collaborative robot arms that could function without massive shielding to protect scientists from the robots. 2nd generation laboratory robots are more versatile than the fixed integrations of 1st generation. 2nd generation systems corresponded with the rise of the automation scheduling software that would manage the workflows of all systems in the modular workflow. In this work, we describe the development and deployment of the 3rd generation of laboratory robotics, or orchestrated robotics. Much like 2nd generation systems, 3rd generation robots enable connections of multiple robotics systems to create complex processes, but 3rd generation systems do not need to be physically connected in the same lab. Instead, they can be integrated across multiple labs or across multiple floors of a building. 3rd generation robots share all actionable sample related data with each other and when paired with a robotics orchestration software suite can even sequence all steps of the process to occur sequentially upon transport of the material to the next automated processing step in the series. With 3rd generation robotics, materials can be delivered via manual or automated methods, dependent upon the throughput needs of the system. In our case study of 3rd generation robotics, we have demonstrated an orchestrated workflow including a Tecan Freedom EVO Robot performing Robocolumn purifications transferring a variable numbers of plates to an Omron autonomous mobile robot (AMR) with a robotic arm. The AMR moves the plates to a BioSero Sample Handling cart, connected to a Hamilton liquid handling robot for liquid handling and finally transferring by AMR again to an Analytics cart for plate reader UV/Vis analysis. This Orchestrated workflow is actively being expanded to include additional robotics equipment to enable robust, lights out biologics processing for development uses.