University of Pennsylvania, Pennsylvania, United States
In this talk, I will begin with discussing the emergence of a whole new field – spatial omics – and then present a new technology platform called DBiT-seq – microfluidic Deterministic Barcoding in Tissue for spatial multi-omics sequencing. It demonstrated co-mapping of whole transcriptome and a large panel of proteins with high spatial resolution. I will also show the latest progress of DBiT as a platform technology to enable, for the first time, spatial profiling of epigenome (histone modifications and chromatin accessibility) at cellular level. Integration with single-cell data allows for accurate cell type annotation in relation to spatial location in tissue. The rise of NGS-based spatial omics is poised to fuel the next wave of biomedical innovation.