The production of complex, multispecific proteins is an important part of modern biopharmaceutical research. Automation of transient transfection of animal cells on a larger scale in suspension cell culture presents a particular challenge. We report on a new continuous cell culture system for transient transfection in 600 ml TubeSpin bioreactors. The system was developed by Sanofi (Frankfurt, DE) and Lab Services (Breda, NL) and is built around a “heavy duty” CoBot on a linear track. Automated shakers, a cell counter and a customized liquid handler in a sterile working environment are fully integrated and software controlled. With the system it is possible to continuously perform cultivation of suspension cells automatically. Further on grow of precultures and transient transfections can be carried out.
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