The Bruker SPR Pro Series instruments are essential tools for achieving optimal performance without compromising on flexibility and throughput. Thus, the Bruker SPR-24 PRO and the Bruker SPR-32 PRO have established themselves as reputable SPR instruments in both the pharmaceutical industry as well as academic research organizations. These instruments exhibit remarkable flexibility by allowing the individual spot addressing thus enabling a wide range of applications, from small molecules to biologics and spanning thermodynamic analysis, kinetic measurements and epitope binning experiments. In this tutorial, we present Bruker Daltonics' latest advancement in SPR instruments, featuring an innovative 8-channel multi spot addressing fluidic design and an exceptional detector, combined with a high-throughput autosampler, as well as automation capabilities - enabling scientists to conduct their work without any concessions. This new and unique instrument allows for the analysis of all types of samples, in a high throughput fashion as well as the possibility of in-depth assay development.