(1115-D) cfDNA Extraction Simplified: An Automated Workflow on Omega Bio-tek’s Cutting-Edge Platform

Abstract: Plasma is a well-known sample type used in the isolation of cell-free DNA (cfDNA) for disease detection, diagnosis, and monitoring. cfDNA consists of small fragments of DNA released by cells as a result of processes such as apoptosis, damage to cells, and the shedding of DNA by normal cells. Leveraging the information stored in cfDNA can revolutionize diagnostics, enabling tests to track cancer progression, assess treatment responses, and customize personalized therapies. Omega Bio-tek has developed a new automated solution to extract cfDNA from 4 mL of human-derived plasma with minimal manual intervention. This revolutionary high-volume nucleic acid purification system used together with prefilled reagent cartridges can extract DNA from up to 24 samples in a single run. Here, we demonstrate this innovative workflow’s capability of extracting cfDNA from human-derived plasma compared to Company T's extraction workflow on their magnetic processor. cfDNA size, quality, and qPCR analyses were performed.