(1204-A) A sustainable open-source biofoundry platform ready for implementation

Abstract: Over 40 countries have national strategies for expanding synthetic biology capacity to build a strong bioeconomy. However, many labs do not have enough funding to secure the software and hardware necessary to function as high throughput biofoundries. Prior to our work, resources for automating customized, in-lab plasmid build workflows were scarce. To fill this gap, we developed a portfolio of open-source lab automation software using OpenTrons OT-2 lab robots. Our tools enable any lab to function as a sustainable biofoundry with minimal upfront investment. First we developed AssemblyTron, which automates DNA assembly, combinatorial mutant library assembly, and modular cloning indexed plasmid library assembly. Next, we developed TidyTron to automate single-use plastic cleaning and reuse. Both platforms have been validated with a series of published tests and screens. As we continue to scale, expand, and implement our biofoundry platform, we are seeking collaborators to perform beta testing in their labs to evaluate and improve platform portability. We also want to provide biofoundry services with our platform, so we need new collaborators that can provide diverse pilot designs for testing and optimizing our workflow.