(1251-D) Corning Matrigel matrix Supplemented Media for Pancreatic Cancer Organoid Assays
Tuesday, February 6, 2024
2:00 PM – 3:00 PM EST
Location: Exhibit Halls AB
Abstract: It has been well demonstrated that organoids better recapitulate the complexity and population diversity of patients as compared to more traditionally used cell lines resulting in the need to develop organoid models for higher throughput and more predictive drug discovery campaigns. One major hurdle to executing these models is the challenging method used to culture organoids which often consists of maintaining cultures in domes of extracellular matrices (ECMs). These ECMs are viscous and temperature sensitive which can be challenging when used in conjunction with automated liquid handling systems. Here, we demonstrate an alternative technique for maintaining organoids in Corning Elplasia microplates using media supplemented with Matrigel matrix. This method allows for the use of a lower concentration of Matrigel matrix in the media making it easier to handle and automate. Additionally, the Elplasia microplate, with its microcavity substrate, allows for significantly more data points per well since organoids are better separated and are in a more uniform focal plane than with dome cultures, making data analysis easier. The combination of the Elplasia microplate and Matrigel matrix supplementation allows for easier image analysis especially when used in conjunction with the Keyence BZ-X Series all-in-one fluorescence microscope. To evaluate this new technique, propidium iodide and nuclear staining were used to quantify organoid viability after drug exposure.