(1154-C) Throughput Meets Flexibility: The Next Generation Multiplexing SPR for Efficient Therapeutic Protein Discovery and Development
Tuesday, February 6, 2024
12:00 PM – 1:00 PM EST
Location: Exhibit Halls AB
Abstract: Surface Plasmon Resonance is an established and widely used biophysical technology in screening and lead development campaigns for novel drug candidates. The real-time, label-free analysis of interactions offers additional insights into kinetics. Multiplexing SPR systems further allow to study the interaction of a drug candidate against multiple targets simultaneously. We developed a novel microfluidic set-up that allows to determine affinity, selectivity and binding mode in a single assay at high throughput. Partnered with our established SPR+ detection system for state-of-the-art sensitivity, the instrument provides multiple assay formats perfectly suited for therapeutic protein discovery and development. We present data from the novel microfluidic set-up proving performance and flexibility. The affinity determination of the established interaction between anti-VEGF antibodies and VEGF 121 and 165 showcases the instrument’s capability to resolve slow off-rates efficiently. We further demonstrate the capacity for multiplexed screening with an interaction in a set of 8 vs 8 interaction partners. Finally, we showcase the flexibility of the instrument for a mode of action analysis in an assay with one interaction measured in eight different buffer conditions simultaneously. We herewith present an SPR microfluidic set-up with industry-leading throughput in affinity determination and multiplexing capabilities for all types of analytes. Thus, the extension of typical parameters from a SPR experiment by a quantitative selectivity assessment allows for a more informed hit selection process in screening campaigns.