Abstract: Within the past few years, many publications advocate the promise of organoids to improve clinical trial success and enable personalized medicine. However, organoid research is suffering from a lack of standardization and high organoid-to-organoid variability. These challenges, together with difficult cultivation techniques are impeding the wider adoption of organoid technologies. To overcome these limitations, we have developed the CellXpress.ai Automated Cell Culture System. This highly integrated organoid generation and cultivation workstation integrates cutting-edge hardware and software technologies with advanced biological science to automate and standardize the 2D and 3D cell culture process. From maintenance, monitoring, and incubation, through imaging, analysis, and data processing, the CellXpress.ai cell culture system delivers consistent, unbiased, and biologically-relevant results at scale. To support scientists at every level of their organoid research, the system guides the user to set up and execute iPSC, tumoroid, and adult stem cell-derived organoid workflows. Key features of theCellXpress.ai cell culture system; (1) Automated cell monitoring using built-in incubation, liquid handling, media exchange, and imaging. (2) Machine learning-assisted automated decision-making to trigger events like feeding, passaging, or compound addition. (3) Automated quality control and sample tracking throughout the entire lifetime of an organoid or iPSC. (4) Minimized human error with assisted protocol creation, cell monitoring, and consumables handling. (5) Unified software package to control hardware, execute workflows, and acquire and analyze data. (6) Newly developed flexible scheduler to support multiple workflows in a multiuser environment and to track the cell journey, consumables usage, and media exchange. As a proof of concept, we demonstrated the successful cultivation of iPSC and human intestinal organoid workflows over multiple passages. The workflows included cell/organoid seeding, feeding, and passaging, with in-line monitoring and machine-learning image analysis and classification. The new CellXpress.ai cell culture system enables researchers to generate high-quality organoids with greater reliability and reproducibility. This addresses the key bottlenecks that come with 3D biology research and will impact the field by allowing the wider adoption of organoids in biopharma and biotech for drug discovery, toxicity studies, and personalized medicine.
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