(1094-C) A solution for reducing carryover signal and improving throughput of fluorescence fragment analysis on microfluidic electrophoresis platform
Tuesday, February 6, 2024
12:00 PM – 1:00 PM EST
Location: Exhibit Halls AB
Abstract: For research use only. Not for use in diagnostic procedures.
Fluorescently labeled nucleic acid fragment analysis (FFA) is an essential tool for a wide variety of applications for enzyme characterization, reaction optimization, and product quality control in molecular bioprocesses. We recently released our FFA120 assay (P/N CLS158081) on LabChip® GX TouchTM I or II, providing an environmental green solution for fluorescently labeled fragment analysis with automated sample loading and quantitative data analysis. There are certain risks of signal carryover from previous samples, especially when sample concentration is high. To avoid the risk of potential signal carryover, the FFA120 assay not only recommends a range of loading sample concentration (20nM to 400nM), but also requires sipper wash steps between test samples (adding sipper wash wells on the plate). It is a robust and conservative solution for solving carryover risks. However, the disadvantage of this solution is also obvious. The throughput is reduced to half, and half of running time is for washing instead of for sample testing. In the study, we optimize microfluidic flow control, report a new FFA120 assay program named “FFA120 v2.asyx”. This new program considers the solvent impact on microfluidic flow rate and optimizes the microfluidic flow control. “FFA120 v2.asyx” reduces the sample carryover signal by at least one order of magnitude without sacrificing assay sensitivity when comparing to the original assay program. Without the sipper washing step, “FFA120 v2.asyx” can analyze full 96 samples in one 96-well plate per run with the current Chip preparation scheme.