(1171-D) High-Throughput Automation of Plasmid DNA Extraction Enhances Efficiency and Reliability
Tuesday, February 6, 2024
2:00 PM – 3:00 PM EST
Location: Exhibit Halls AB
Abstract: The isolation of plasmid DNA is an important molecular biology procedure for many downstream applications. The multi-step protocol is simple, but labor-intensive and error prone. In this study, we present an optimized approach for plasmid DNA extraction using Promega's Wizard MagneSil TfxTM paramagnetic particles and Analytik Jena's CyBio FeliX liquid changer to create a fully automated protocol for medium-throughput screening applications. Escherichia coli JM109 bacteria, were transformed with pGL4.50 and cultured in Terrific Broth (TB) or Luria Broth (LB) medium. The subsequent extraction was performed on the CyBio FeliX. Spectrophotometry and gel electrophoresis were used to determine DNA concentrations, absorbance ratio and plasmid size. The TB culture yielded higher plasmid amounts, which can be attributed to the higher culture density. The results show that the automated procedure significantly reduced processing time and minimized variability while maintaining high quality and purity of plasmid DNA. This study highlights the effectiveness of the Promega MagneSil Tfx™ System and Analytik Jena CyBio FeliX in the simple, rapid, and reliable isolation of transfection-quality plasmid DNA in a 96-well high-throughput format.