(1337-B) Lumit™ Cytokine Immunoassay Automation for High-Throughput Screening
Monday, February 5, 2024
2:00 PM – 3:00 PM EST
Location: Exhibit Halls AB
Abstract: Accurate detection of cytokine levels in cell culture models is crucial for advancing inflammation and immunology research, including the discovery and development of treatments for autoimmune and inflammatory diseases, as well as cancer therapies involving small molecules, biologics, and cell therapies. Lumit™ technology introduces a rapid, luminescent method for cytokine detection, ideal for high-throughput screening. These fully homogeneous Lumit™ cytokine immunoassays eliminate the need for sample transfer or wash steps and are adaptable for automation in 384-well plate format. The assays not only offer a short turnaround time of approximately 70 minutes but also utilize optimized volumes compatible with diverse liquid dispensing instruments. Noteworthy is their exceptional sensitivity, with a minimum detectable dose (MDD) under 10 pg/mL, and a broad linear range extending over three logs of cytokine concentration, significantly reducing the necessity for sample dilution. Our current study involved the use of automated liquid handling both to plate and treat human peripheral blood mononuclear cells (PBMCs) in 384-well format, as well as in the subsequent application of Lumit™ cytokine immunoassays for assessment of IL-2 and IFN-γ release. The standard curves for both cytokines demonstrated remarkable linearity and stable glow kinetics, with assay signal half-lives around 2 hours. Optimizing the number of PBMCs per well enabled us to achieve dose-response curves for IL-2 and IFN-γ release in response to Cell Stimulation Cocktail (CSC) treatment with signal-to-background (S/B) ratios exceeding 400-fold. Important for batch plate processing, we observed consistent S/B performance sustained for at least 2 hours post-signal generation. In summary, Lumit™ cytokine immunoassays represent a streamlined, rapid, and effective methodology for cytokine detection. Their simplicity and adaptability for automation make them highly suitable for large-scale, high-throughput screening applications.