(1033-B) Extracellular ATP measurement in real time using live cells
Monday, February 5, 2024
2:00 PM – 3:00 PM EST
Location: Exhibit Halls AB
Abstract: When you think about ATP, the energy-rich molecule essential for life, you are thinking about the ATP that is inside of cells. ATP outside of cells or extracellular ATP is an important signaling molecule involved in cell proliferation and other processes. Interest in extracellular ATP is rising due to its role in promoting the immunogenic cell death (ICD) response. Cells that undergo ICD-driven apoptosis release extracellular ATP and other molecules known as damage associated molecular pattern (DAMP) molecules. Working together extracellular ATP and other DAMP molecules promote the innate and adaptive immune responses. It is hoped that new drugs can be discovered that stimulate extracellular ATP release by cells and subsequently drive these cells to undergo apoptosis by ICD for therapeutic purposes. Current measurements of extracellular ATP involve collection and processing of supernatant followed by end-point measurements making the process time and resource consuming. Here we present results that use a simple add-mix-measure assay optimized to detect extracellular ATP from living cells in real time. The results show distinct patterns of extracellular ATP release among potential ICD inducers.