(1268-A) Development of a High Throughput Cell Based Activity Screen to Identify Inhibitors of a Complex First in Class Redox Enzyme using Acoustic Ejection Mass Spectrometry (AEMS)
Monday, February 5, 2024
12:00 PM – 1:00 PM EST
Location: Exhibit Halls AB
Abstract: Mass spectrometry (MS) has found widespread utility in numerous aspects of biopharmaceutical R&D; however, throughput of traditional liquid chromatography MS has limited application to high-throughput (HT) in vitro pharmacology screening, especially large file primary HTS. Recently developed acoustic ejection coupled to mass spectrometry (AEMS) provides very fast (~1 Hz) contactless sampling from a wide range of matrices, enabling analysis of label-free assays on a timescale orders of magnitude faster than LC-MS, and similar to that of state-of-the-art plate readers. Integrating AEMS into high throughput pharmacology assays can reduce assay cost, ameliorate confounding interference introduced by fluorescent probes, and largely bypass time-consuming method development. Taken together these attributes have the promise of accelerating early discovery operations and project progression. This poster will demonstrate the utility and enablement of the AEMS system in a cell-based activity assay providing simultaneous readouts for both substrate and product of a complex first in class redox enzyme. Analysis of a 384-well plate requires only 8 minutes and with this throughput, primary and dose-response screening of numerous subsets was successfully completed.